A synthetic RNA-mediated evolution system in yeast.
Jensen ED, Laloux M, Lehka BJ, Pedersen LE, Jakociunas T, Jensen MK, Keasling JD
Nucleic Acids Res. 2021 Sep 7;49(15):e88. doi: 10.1093/nar/gkab472.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 177269 | pEDJ400 | USER cloning-compatible 2μ vector (URA3) |
| 177270 | pEDJ437 | USER cloning-compatible 2μ vector (HIS3) |
| 177271 | pEDJ8 | Double gRNA for RNH1 RNH201 knock-out |
| 177272 | pEDJ333 | GAL1p:Cas9:CYC1t |
| 177273 | pMLB10 | GAL1p:T7RNAP(F11L;T613A):CYC1t |
| 177274 | pEDJ506 | ADH1t:HIS3_23∆29-XII-5 :<-PGK1p |
| 177275 | pEDJ508 | ADH1t:T7p:cgRNA_HIS3_stop:tZ |