PEAR, a flexible fluorescent reporter for the identification and enrichment of successfully prime edited cells.

Simon DA, Talas A, Kulcsar PI, Biczok Z, Krausz SL, Varady G, Welker E
Elife. 2022 Feb 23;11. pii: 69504. doi: 10.7554/eLife.69504. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
80453	pX330-Flag-wtSpCas9-H840A	SpCas9 mammalian expression vector
177178	pDAS12125_PEAR-GFP	PEAR-GFP target plasmid with split EGFP and disrupted 5' splice site
177179	pDAS12124_PEAR-GFP-preedited	PEAR-GFP control plasmid with split EGFP and intact 5' splice site
177180	pDAS12069_U6-pegRNA-mCherry	pegRNA cloning plasmid with mCherry marker
177181	pDAS12222_U6-pegRNA-BFP	pegRNA cloning plasmid with BFP marker
177182	pDAS12230_pegRNA-PEAR-GFP(10PBS-24RT)-mCherry	plasmid expressing a pegRNA targeting the PEAR-GFP plasmid along with an mCherry marker
177183	pDAS12137_sgRNA-PEAR-GFP-nick(+17)-mCherry	plasmid expressing an sgRNA targeting the PEAR-GFP plasmid along with an mCherry marker
177184	pDAS12342_PEAR-GFP_2in1	PEAR-GFP plasmid with a pegRNA that targets its own plasmid
177185	pDAS12395_PEAR-GFP_2.0-preedited	PEAR-GFP3 control plasmid with split EGFP and intact 5' splice site
177186	pDAS12489_PEAR-GFP_2in1_2.0	PEAR-GFP3 plasmid with a pegRNA that targets its own plasmid