A Tunable and Expandable Transactivation System in Probiotic Yeast Saccharomyces boulardii.
Kwak S, Mahmud B, Dantas G
ACS Synth Biol. 2022 Jan 21;11(1):508-514. doi: 10.1021/acssynbio.1c00384. Epub 2021 Dec 23.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 177155 | pRS42H_PTEF1_dCas9_TCYC1 | Expresses dCas9 in yeast cells |
| 177156 | pRS42K_PCP-VP64_A-PP7 | Expresses transactivator (PCP-VP64) and scRNA (A-PP7) in yeast cells |
| 177157 | pRS42K_PCP-VP64_B-PP7 | Expresses transactivator (PCP-VP64) and scRNA (B-PP7) in yeast cells |
| 177160 | pRS42K_MCP-VP64_C-MS2 | Expresses transactivator (MCP-VP64) and scRNA (C-MS2) in yeast cells |
| 177161 | pRS42K_MCP-VP64_D-MS2 | Expresses transactivator (MCP-VP64) and scRNA (D-MS2) in yeast cells |
| 177162 | pRS426-PGAL7-A_GFP_TCYC1 | Expresses yeGFP under the control of a synthetic promoter (PGAL7-A) |
| 177163 | pRS426-PGAL7-B_GFP_TCYC1 | Expresses yeGFP under the control of a synthetic promoter (PGAL7-B) |
| 177164 | pRS426-PGAL7-C_RFP_TCYC1 | Expresses yeRFP under the control of a synthetic promoter (PGAL7-C) |
| 177165 | pRS426-PGAL7-D_RFP_TCYC1 | Expresses yeRFP under the control of a synthetic promoter (PGAL7-D) |