A multiplexed epitope barcoding strategy that enables dynamic cellular phenotypic screens.
Kudo T, Lane K, Covert MW
Cell Syst. 2022 May 18;13(5):376-387.e8. doi: 10.1016/j.cels.2022.02.006. Epub 2022 Mar 21.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 177149 | in-frame kanamycin backbone | Expresses kanamycin when ligated in-frame |
| 177197 | in-frame ampicillin PKAKTR backbone | PKAKTR mutagenesis template with in-frame ampicillin site |
| 177198 | pLenti EPICode screen backbone | lentiviral vector backbone for both barcodes and KTR |
| 177199 | pENTR PKAKTRn-Clover | Gateway entry vector encoding PKAKTRnew-Clover |
| 177200 | pLenti PKAKTRn-Clover | Lentiviral vector encoding PKAKTRnew-Clover |