Reconstitution and use of highly active human CDK1:Cyclin-B:CKS1 complexes.
Huis In 't Veld PJ, Wohlgemuth S, Koerner C, Muller F, Janning P, Musacchio A
Protein Sci. 2021 Nov 17. doi: 10.1002/pro.4233.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 177011 | pLIB-GST-3C-CDK1 | Generate baculovirus for insect cell expression of CDK1 with N-terminal GST-3C (PreScission) |
| 177012 | pLIB-HIS-TEV-Cyclin-B | Generate baculovirus for insect cell expression of Cyclin-B with N-terminal Polyhistidine-TEV |
| 177013 | pLIB-HIS-TEV-CKS1 | Generate baculovirus for insect cell expression of CKS1 with N-terminal Polyhistidine-TEV |
| 177014 | pLIB-HIS-TEV-scCAK1 | Generate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEV |