Highly Efficient Genome Editing in Plant Protoplasts by Ribonucleoprotein Delivery of CRISPR-Cas12a Nucleases.
Zhang Y, Cheng Y, Fang H, Roberts N, Zhang L, Vakulskas CA, Niedz RP, Culver JN, Qi Y
Front Genome Ed. 2022 Jan 31;4:780238. doi: 10.3389/fgeed.2022.780238. eCollection 2022.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 176889 | pYPQ220-Ultra | Gateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoter |
| 176890 | pYPQ230-E795L | Gateway entry plasmid (attL1 & attR5) expressing LbCas12a with E795L mutation, without promoter |