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Direct delivery and fast-treated Agrobacterium co-culture (Fast-TrACC) plant transformation methods for Nicotiana benthamiana.

Cody JP, Maher MF, Nasti RA, Starker CG, Chamness JC, Voytas DF
Nat Protoc. 2022 Oct 17. pii: 10.1038/s41596-022-00749-9. doi: 10.1038/s41596-022-00749-9. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
176863pTAG1First sgRNA in a tRNA separated array of sgRNAs
176864pTAG2First sgRNA in a tRNA separated array of sgRNAs
176865pTAG2EFirst sgRNA in a tRNA separated array of sgRNAs
176866pTAG3First sgRNA in a tRNA separated array of sgRNAs
176867pTAG4First sgRNA in a tRNA separated array of sgRNAs
176868pTAG4EFirst sgRNA in a tRNA separated array of sgRNAs
176869pTAG5First sgRNA in a tRNA separated array of sgRNAs
176870pTAG6EFirst sgRNA in a tRNA separated array of sgRNAs
176871pMC-1-AtUbi10-6MoClo Level 0 plasmid, contains AtUbi1 promoter
176872pMC-1-CmYLCV-6MoClo Level 0 plasmid, contains CmYLCV-6 promoter
176873pMOD_A4800ECEmpty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit
176874pMOD_B4800ECEmpty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit
176875pMOD_C'4800ECEmpty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit
176876pMOD_D4800ECEmpty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit
176877pMOD_A5801High constitutive expression of Firefly Luciferase in plants
176878pMOD_A5803High constitutive expression of Firefly Luciferase in plants
176879pMOD_C'5802High constitutive expression of Firefly Luciferase in plants
176880pMOD_D5802High constitutive expression of Firefly Luciferase in plants
176881pMC-9-35S-11MoClo Level 0 plasmid, contains 35S
176882pMC-9-HSP-11MoClo Level 0 plasmid, contains HSP
176883pJC225MoClo Level1 plasmid, allows transition into Cermak, et al., 2017 GG cloning system
176884pRN119Encodes STM and WUS2 for ectopic meristem induction in plants

Antibodies from Article