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Optimized APEX2 peroxidase-mediated proximity labeling in fast- and slow-growing mycobacteria.

Ahamed M, Jaisinghani N, Li M, Winkeler I, Silva S, Previti ML, Seeliger JC
Methods Enzymol. 2022;664:267-289. doi: 10.1016/bs.mie.2021.11.021. Epub 2021 Dec 30. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
176842pRibo-APEX2mExpression of APEX2 from a codon-optimized gene (multi-copy episomal plasmid)
176843pRiboI-APEX2mExpression of APEX2 from a codon-optimized gene (single-copy integrating plasmid)
176844pRibo-Sec-APEX2mExpression of APEX2 in the periplasm from a codon-optimized gene (multi-copy episomal plasmid)
176845pRiboI-Sec-APEX2mExpression of APEX2 in the periplasm from a codon-optimized gene (single-copy integrating plasmid)

Antibodies from Article