Optimized APEX2 peroxidase-mediated proximity labeling in fast- and slow-growing mycobacteria.
Ahamed M, Jaisinghani N, Li M, Winkeler I, Silva S, Previti ML, Seeliger JC
Methods Enzymol. 2022;664:267-289. doi: 10.1016/bs.mie.2021.11.021. Epub 2021 Dec 30.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
176842 | pRibo-APEX2m | Expression of APEX2 from a codon-optimized gene (multi-copy episomal plasmid) |
176843 | pRiboI-APEX2m | Expression of APEX2 from a codon-optimized gene (single-copy integrating plasmid) |
176844 | pRibo-Sec-APEX2m | Expression of APEX2 in the periplasm from a codon-optimized gene (multi-copy episomal plasmid) |
176845 | pRiboI-Sec-APEX2m | Expression of APEX2 in the periplasm from a codon-optimized gene (single-copy integrating plasmid) |