Addgene: Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos.

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Norbert Perrimon
Viswanatha et al

Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos.

Viswanatha R, Mameli E, Rodiger J, Merckaert P, Feitosa-Suntheimer F, Colpitts TM, Mohr SE, Hu Y, Perrimon N
Nat Commun. 2021 Nov 24;12(1):6825. doi: 10.1038/s41467-021-27129-3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
176682	pSL1180-HR-PUbEBFP2-NLS	Expression of nuclear localized EBFP2 under Ae. aegypti Polyubiquitin promoter (AAEL003888), EBFP2 version of pSL1180-HR-PUbECFP (Addgene #47917)
176681	pSL1180-HR-PUbEGFP-NLS	Expression of nuclear localized EGFP under Ae. aegypti Polyubiquitin promoter (AAEL003888), EGFP version of pSL1180-HR-PUbECFP (Addgene #47917)
176680	pAaePUb::Cas9-2A-Neo	Expression of Drosophila codon optimized spCas9 under Ae. aegypti Polyubiquitin promoter (AAEL003888)
176679	pDmAct5C::Cas9-2A-Neo	Expression of Drosophila codon optimized spCas9 under Drosophila Act5C promoter
176678	pLib6.4B-Consensus	Expression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro\|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism
176676	pLib6.4B-Aalb_743	Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro\|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism
176675	pLib6.4B-Aalb_726	Expression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro\|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism
176674	pLib6.4B-Aaeg_972	Expression of sgRNA under Ae. Aegypti U6 (AAEL028972) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro\|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism
176673	pLib6.4B-Aaeg_774	Expression of sgRNA under Ae. Aegypti U6 (AAEL017774) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro\|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism
176672	pLib6.4B-Aaeg_763	Expression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro\|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Antibodies from Article