Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos.
Viswanatha R, Mameli E, Rodiger J, Merckaert P, Feitosa-Suntheimer F, Colpitts TM, Mohr SE, Hu Y, Perrimon N
Nat Commun. 2021 Nov 24;12(1):6825. doi: 10.1038/s41467-021-27129-3.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 176652 | pLib6.6 | Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176653 | pLib6.4-Agam_557 | Expression of sgRNA under An. gambiae U6-1 (AGAP013557) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176654 | pLib6.4-Agam_695 | Expression of sgRNA under An. gambiae U6-2 (AGAP013695) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176655 | pLib6.4-Cquin_596 | Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176656 | pLib6.4-Cquin_728 | Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039728) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176657 | pLib6.4-Cquin_801 | Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039801) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176658 | pLib6.4-Aaeg_763 | Expression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176659 | pLib6.4-Aaeg_774 | Expression of sgRNA under Ae. Aegypti U6 (AAEL017774) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176660 | pLib6.4-Aaeg_972 | Expression of sgRNA under Ae. Aegypti U6 (AAEL028972) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176661 | pLib6.4-Aalb_726 | Expression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176662 | pLib6.4-Aalb_743 | Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176663 | pLib6.4-Aalb_744 | Expression of sgRNA under Ae. albopictus U6 (AALF029744) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176664 | pLib6.4-Consensus | Expression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176665 | pLib6.4B | Expression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176666 | pLib6.6B | Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176667 | pLib6.4B-Agam_557 | Expression of sgRNA under An. gambiae U6-1 (AGAP013557) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176668 | pLib6.4B-Agam_695 | Expression of sgRNA under An. gambiae U6-2 (AGAP013695) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176669 | pLib6.4B-Cquin_596 | Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176670 | pLib6.4B-Cquin_728 | Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039728) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176671 | pLib6.4B-Cquin_801 | Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039801) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176672 | pLib6.4B-Aaeg_763 | Expression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176673 | pLib6.4B-Aaeg_774 | Expression of sgRNA under Ae. Aegypti U6 (AAEL017774) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176674 | pLib6.4B-Aaeg_972 | Expression of sgRNA under Ae. Aegypti U6 (AAEL028972) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176675 | pLib6.4B-Aalb_726 | Expression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176676 | pLib6.4B-Aalb_743 | Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176678 | pLib6.4B-Consensus | Expression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism |
| 176679 | pDmAct5C::Cas9-2A-Neo | Expression of Drosophila codon optimized spCas9 under Drosophila Act5C promoter |
| 176680 | pAaePUb::Cas9-2A-Neo | Expression of Drosophila codon optimized spCas9 under Ae. aegypti Polyubiquitin promoter (AAEL003888) |
| 176681 | pSL1180-HR-PUbEGFP-NLS | Expression of nuclear localized EGFP under Ae. aegypti Polyubiquitin promoter (AAEL003888), EGFP version of pSL1180-HR-PUbECFP (Addgene #47917) |
| 176682 | pSL1180-HR-PUbEBFP2-NLS | Expression of nuclear localized EBFP2 under Ae. aegypti Polyubiquitin promoter (AAEL003888), EBFP2 version of pSL1180-HR-PUbECFP (Addgene #47917) |