Synthetic multistability in mammalian cells.

Zhu R, Del Rio-Salgado JM, Garcia-Ojalvo J, Elowitz MB
Science. 2022 Jan 21;375(6578):eabg9765. doi: 10.1126/science.abg9765. Epub 2022 Jan 21. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
176624	MF64_PB-TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA	TF B self-activation cassette in MultiFate-2.1 and MultiFate-2.3
176626	MF82_PB-TRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA	TF A self-activation cassette in MultiFate-2.2 and MultiFate-3
176627	MF83_PB-TRE3G-6x42bs-10x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA	TF B self-activation cassette in MultiFate-2.2 and MultiFate-3
176628	MF84_PB-14xUAS-6x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA	TF A self-activation cassette in MultiFate-2.3
176629	MF85_PB-14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA	TF C self-activation cassette in MultiFate-3
176630	MF87_PB-EF1a-TET3G-P2A-ERT2-Gal4-BGHpA	Integrated in CHO-K1 to make parental polyclonal lines for all other MultiFate cell lines