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Generation of scalable cancer models by combining AAV-intron-trap, CRISPR/Cas9, and inducible Cre-recombinase.

Boddu PC, Gupta AK, Kim JS, Neugebauer KM, Waldman T, Pillai MM
Commun Biol. 2021 Oct 13;4(1):1184. doi: 10.1038/s42003-021-02690-1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
176590pAAV_FLAG_hSF3B1Adenoviral construct to insert a N-terminal FLAG to one allele of human SF3B1
176591pAAV_K700E_hSF3B1Adenoviral construct to change K to E at 700th amino acid (K700E) in human SF3B1
176592pAAV_S34F_U2AF1Adenoviral construct to change S to F at 34th amino acid (S34F) in human U2AF1

Antibodies from Article