Differentially Optimized Cell-Free Buffer Enables Robust Expression from Unprotected Linear DNA in Exonuclease-Deficient Extracts.

Batista AC, Levrier A, Soudier P, Voyvodic PL, Achmedov T, Reif-Trauttmansdorff T, DeVisch A, Cohen-Gonsaud M, Faulon JL, Beisel CL, Bonnet J, Kushwaha M
ACS Synth Biol. 2022 Jan 16. doi: 10.1021/acssynbio.1c00448. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
176580	BL21 ΔrecB	E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression.
176581	BL21 ΔrecBCD	E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression.
176582	BL21 Rosetta2 ΔrecB	E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.
176583	BL21 Rosetta2 ΔrecBCD	E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.
176584	BL21 Rosetta2 GamS	E. coli BL21 strain for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.
176585	BL21 Rosetta2 ΔrecB GamS	E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.
176586	BL21 Rosetta2 ΔrecBCD GamS	E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.