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Non-invasive and high-throughput interrogation of exon-specific isoform expression.

Truong DJ, Phlairaharn T, Esswein B, Gruber C, Tumen D, Baligacs E, Armbrust N, Vaccaro FL, Lederer EM, Beck EM, Geilenkeuser J, Goppert S, Krumwiede L, Gratz C, Raffl G, Schwarz D, Zirngibl M, Zivanic M, Beyer M, Korner JD, Santl T, Evsyukov V, Strauss T, Schwarz SC, Hoglinger GU, Heutink P, Doll S, Conrad M, Giesert F, Wurst W, Westmeyer GG
Nat Cell Biol. 2021 Jun;23(6):652-663. doi: 10.1038/s41556-021-00678-x. Epub 2021 Jun 3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
177865pEXSISERS_NLuc_FRT-PuroRCloning Backbone for NanoLuc-luciferase-based EXSISERS containing FRT-sites flanked PuroR cassette
177866pEXSISERS_FLuc_loxP-PuroRCloning Backbone for Firefly-luciferase-based EXSISERS containing loxP-sites flanked PuroR cassette
177867pEXSISERS_BSD_FRT-PuroRCloning Backbone for Blasticidin-S-deaminase-based EXSISERS containing FRT-sites flanked PuroR cassette
177868pEXSISERS_mGreenLantern_FRT-PuroR-HSV-TKCloning Backbone for mGreenLantern-based EXSISERS containing FRT-sites flanked PuroR-HSV-TK cassette; clone homology arms via BbsI (or BpiI).
177869pEXSISERS_SurfaceHaloTag_FRT-PuroRCloning Backbone for Surface-HaloTag-based EXSISERS containing FRT-sites flanked PuroR cassette; clone homology arms via BbsI (or BpiI).

Antibodies from Article