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VPS13D promotes peroxisome biogenesis.

Baldwin HA, Wang C, Kanfer G, Shah HV, Velayos-Baeza A, Dulovic-Mahlow M, Bruggemann N, Anding A, Baehrecke EH, Maric D, Prinz WA, Youle RJ
J Cell Biol. 2021 May 3;220(5). pii: 212018. doi: 10.1083/jcb.202001188. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
176462pGFP-Ub-VPS13Dexpressing GFP-Ub marked VPS13D in mammalian cells. GFP-Ub will be cleaved by DUBs to express untagged VPS13D
176463pGFP-Ub-VPS13D N3521Sexpressing VPS13D patient mutant N3521S in mammalian cells
176464pVPS13D-I-EGFPexpress internally GFP-tagged VPS13D in mammalian cells
176465pGFP-Ub-VPS13D_dNexpress VPS13D lacking N-terminal 117 aa in mammalian cells
176466pGFP-Ub-VPS13D_dCexpress VPS13D lacking C-terminal 407 aa in mammalian cells

Antibodies from Article