Designer membraneless organelles sequester native factors for control of cell behavior.
Garabedian MV, Wang W, Dabdoub JB, Tong M, Caldwell RM, Benman W, Schuster BS, Deiters A, Good MC
Nat Chem Biol. 2021 Sep;17(9):998-1007. doi: 10.1038/s41589-021-00840-4. Epub 2021 Aug 2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 176395 | Yiplac211 proGAL1-TsCC(A)-RGG-GFP-RGG-RGG | Expresses GFP tagged triple RGG scaffold with N-terminal TsCC(A) coiled coil. Expression induced in media containing galactose. |
| 176396 | Yiplac211 proGAL1-SZ1-RGG-GFP-RGG-RGG | Expresses GFP tagged triple RGG scaffold with N-terminal SynZip1 coiled coil (Sz1). Expression induced in media containing galactose. |
| 176397 | pfa6a mScarlet-TsCC(B)::KANMX | Plasmid for cloning mScarlet fused to TsCC(B) coiled coil into yeast. |
| 176400 | Yiplac211 proGAL1-FRB-RGG-GFP-RGG-RGG | Expresses GFP tagged triple RGG scaffold with N-terminal FRB. Expression induced in media containing galactose. |
| 185905 | pfa6a mScarlet-FKBP::KANMX | Plasmid for cloning mScarlet fused to FKBP into yeast. |
| 185906 | pfa6a PhoCl-TsCC(B)::KANMX | Plasmid for cloning PhoCl fused to TsCC(B) into yeast. |