Compact RNA editors with small Cas13 proteins.
Kannan S, Altae-Tran H, Jin X, Madigan VJ, Oshiro R, Makarova KS, Koonin EV, Zhang F
Nat Biotechnol. 2021 Aug 30. pii: 10.1038/s41587-021-01030-2. doi: 10.1038/s41587-021-01030-2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 176314 | pAB1619 hU6-BpiI-Cas13bt1-DR | hU6-BpiI-Cas13bt1-DR (crRNA expression) |
| 176315 | pAB1620 hU6-BpiI-Cas13bt3-DR | hU6-BpiI-Cas13bt3-DR (crRNA expression) |
| 176316 | pAB1678 CMV-HIVNES-GS-Cas13bt1 | CMV-HIVNES-GS-Cas13bt1 (human codon optimized Cas13bt1 expression) |
| 176317 | pAB1679 CMV-HIVNES-GS-Cas13bt3 | CMV-HIVNES-GS-Cas13bt3 (human codon optimized Cas13bt3 expression) |
| 176319 | pAB1676 REPAIR.t1 | CMV-HIVNES-GS-dCas13bt1- (GGS)2-huADAR2dd(E488Q) (REPAIR.t1 expression) |
| 176320 | pAB1677 REPAIR.t3 | CMV-HIVNES-GS-dCas13bt3- (GGS)2-huADAR2dd(E488Q) (REPAIR.t3 expression) |
| 176321 | pAB1923 REPAIR.t1-S | CMV-HIVNES-GS-dCas13bt1- (GGS)2- huADAR2dd(E488Q/E620G/Q696L) (REPAIR.t1-S expression, specificity enhancing mutations) |
| 176323 | pAB2076 pAAV REPAIR.t1 | pAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production) |