CRISPR/Cas13 effectors have differing extents of off-target effects that limit their utility in eukaryotic cells.
Ai Y, Liang D, Wilusz JE
Nucleic Acids Res. 2022 Mar 4. pii: 6542487. doi: 10.1093/nar/gkac159.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 176299 | Hy_pMtnA FFLuc SV40 | Expresses firefly luciferase (FFLuc) mRNA from the Drosophila Metallothionein A promoter |
| 176300 | Hy_pUbi-p63e mCherry SV40 | Expresses mCherry mRNA from the Drosophila Ubi-p63e promoter |
| 176301 | pcDNA3.1(+) mCherry | Expresses mCherry mRNA from CMV promoter |
| 176302 | Hy_pMtnA mCherry SV40 | Expresses mCherry mRNA from the Drosophila Metallothionein A promoter |
| 176303 | pUb RxCas13d + RxCas13d guide RNA | Expresses RxCas13d and its associated guide RNA in Drosophila cells |
| 176304 | pUb dRxCas13d + RxCas13d guide RNA | Expresses catalytic dead RxCas13d and its associated guide RNA in Drosophila cells |
| 176305 | pUb PspCas13b + PspCas13b guide RNA | Expresses PspCas13b and its associated guide RNA in Drosophila cells |
| 176306 | pUb dPspCas13b + PspCas13b guide RNA | Expresses catalytic dead PspCas13b and its associated guide RNA in Drosophila cells |
| 176307 | pUb LwaCas13a + LwaCas13a Guide RNA | Expresses LwaCas13a and its associated guide RNA in Drosophila cells |