Targeting DNA polymerase to DNA double-strand breaks reduces DNA deletion size and increases templated insertions generated by CRISPR/Cas9.

Yoo KW, Yadav MK, Song Q, Atala A, Lu B
Nucleic Acids Res. 2022 Mar 22. pii: 6552087. doi: 10.1093/nar/gkac186. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
176234	pspCas9-POLI-ST2-com vector	Vector plasmid for expressing spCas9-POLI fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-POLI to enhance expression. The ST2 loop of the sgRNA scaffold was replace
176235	pspCas9-Klenow-ST2-com-vector	Vector plasmid for expressing spCas9-Klenow fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-Klenow to enhance expression.
176236	pspCas9-3exo-Tetra-com-CLCN5-sp-g1	Plasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.
176237	pspCas9-5Exo-tetra-com-hCLCN5-sp-g1	Plasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.
176238	pspCas9-Exo-tetra-com-hCLCN5-sp-g1	Plasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.