A cell-free nanobody engineering platform rapidly generates SARS-CoV-2 neutralizing nanobodies.
Chen X, Gentili M, Hacohen N, Regev A
Nat Commun. 2021 Sep 17;12(1):5506. doi: 10.1038/s41467-021-25777-z.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 176208 | pBx1-VHH template1-3XMyc-Spacer | PCR template for generating CeVICA VHH/nanobody library |
| 176209 | pBx1-VHH template2-3XMyc-Spacer | PCR template for generating CeVICA VHH/nanobody library |
| 176210 | pBx1-VHH template3-3XMyc-Spacer | PCR template for generating CeVICA VHH/nanobody library |
| 176211 | pETx-SR6c3 | Bacterial expression of the SR6c3 nanobody targeting the receptor binding domain of SARS-CoV-2 spike protein |
| 176212 | pETx-SR6v15 | Bacterial expression of the SR6v15 nanobody targeting the receptor binding domain of SARS-CoV-2 spike protein |
| 176213 | pETx-SR6v15.d | Bacterial expression of the SR6v15.d targeting the receptor binding domain of SARS-CoV-2 spike protein |
| 176214 | pETx-SR6v15.t | Bacterial expression of the SR6v15.t targeting the receptor binding domain of SARS-CoV-2 spike protein |