A cell-free nanobody engineering platform rapidly generates SARS-CoV-2 neutralizing nanobodies.

Chen X, Gentili M, Hacohen N, Regev A
Nat Commun. 2021 Sep 17;12(1):5506. doi: 10.1038/s41467-021-25777-z. PubMed Article

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ID	Plasmid	Purpose
176214	pETx-SR6v15.t	Bacterial expression of the SR6v15.t targeting the receptor binding domain of SARS-CoV-2 spike protein
176213	pETx-SR6v15.d	Bacterial expression of the SR6v15.d targeting the receptor binding domain of SARS-CoV-2 spike protein
176212	pETx-SR6v15	Bacterial expression of the SR6v15 nanobody targeting the receptor binding domain of SARS-CoV-2 spike protein
176211	pETx-SR6c3	Bacterial expression of the SR6c3 nanobody targeting the receptor binding domain of SARS-CoV-2 spike protein
176210	pBx1-VHH template3-3XMyc-Spacer	PCR template for generating CeVICA VHH/nanobody library
176209	pBx1-VHH template2-3XMyc-Spacer	PCR template for generating CeVICA VHH/nanobody library
176208	pBx1-VHH template1-3XMyc-Spacer	PCR template for generating CeVICA VHH/nanobody library