Escherichia coli O127 group 4 capsule proteins assemble at the outer membrane.
Larson MR, Biddle K, Gorman A, Boutom S, Rosenshine I, Saper MA
PLoS One. 2021 Nov 15;16(11):e0259900. doi: 10.1371/journal.pone.0259900. eCollection 2021.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 176191 | pSA10(gfcD) | gfcD protein expression |
| 176192 | pSA10(gfcD-10xHis) | gfcD protein expression |
| 176193 | pSA10(gfcBCD) | expresses 3 overlapping genes (gfcB, gfcC, gfcD) from this one operon |
| 176194 | pSA10(gfcB-His-CD) | expresses 3 overlapping genes (gfcB-6xHis, gfcC, gfcD) from this one operon |
| 176196 | pMCSG7(gfcB) | gfcB protein expression |
| 176197 | pMCSG7(gfcC) | gfcC protein expression |
| 176198 | pMCSG26(gfcD) | gfcD protein expression |
| 176199 | pBH31(gfcD) | Encodes pelC signal sequence followed by His6-GfcD. Should secrete to periplasm |
| 176200 | pBH31(gfcD-10xHis) | Encodes pelC signal sequence followed by His10-GfcD. Should secrete to periplasm |