Tension promotes kinetochore-microtubule release by Aurora B kinase.
Chen GY, Renda F, Zhang H, Gokden A, Wu DZ, Chenoweth DM, Khodjakov A, Lampson MA
J Cell Biol. 2021 Jun 7;220(6). pii: 212027. doi: 10.1083/jcb.202007030.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 176201 | CAG.mCherry-eDHFR | Negative control of targeted optogenetic manipulation using TMP-Halo dimerization system |
| 176202 | CAG.mCherry-INBox-eDHFR | Single INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization system |
| 176203 | CAG.INBox-mCherry-INBox-eDHFR | Double INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization system |
| 176204 | CAG.MCAK-mCherry-eDHFR | MCAK (179-583) recruitment responsible for targeted microtubule depolymerization using TMP-Halo dimerization system |
| 176205 | CAG.CenpB(FL)-GFP-Halo | Centromeric anchor for TMP-Halo dimerization system |
| 176206 | CAG.3xHalo-GFP-SPC25 | Kinetochore anchor for TMP-Halo dimerization system |
| 176207 | CAG.mCherry-INBox(AAA)-eDHFR | INBox TSS(892-894)-mutant replaced by AAA responsible for targeted Aurora B recruitment without activation using TMP-Halo dimerization system |