Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based Applications, Genomic Integrations and Combinatorial Libraries.
Otto M, Skrekas C, Gossing M, Gustafsson J, Siewers V, David F
ACS Synth Biol. 2021 Dec 17;10(12):3461-3474. doi: 10.1021/acssynbio.1c00408. Epub 2021 Dec 3.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 176152 | pMC-X2 | preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-2 |
| 176153 | pMC-X3 | preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-3 |
| 176154 | pMC-X4 | preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-4 |
| 176155 | pMC-XI1 | preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-1 |
| 176156 | pMC-XI2 | preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-2 |
| 176157 | pMC-XI3 | preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-3 |
| 176158 | pMC-XI5 | preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-5 |
| 176159 | pMC-XII1 | preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-1 |
| 176160 | pMC-XII2 | preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-2 |
| 176161 | pMC-XII5 | preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-5 |
| 176162 | pMC8b-X2up | yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site X-2 |
| 176163 | pMC7-X2dw | yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site X-2 |
| 176164 | pMC8b-X4up | yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site X-4 |
| 176165 | pMC7-X4dw | yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site X-4 |
| 176166 | pMC8b-XI1up | yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-1 |
| 176167 | pMC7-XI1dw | yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-1 |
| 176168 | pMC8b-XI2up | yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-2 |
| 176169 | pMC7-XI2dw | yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-2 |
| 176170 | pMC8b-XI3up | yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-3 |
| 176171 | pMC7-XI3dw | yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-3 |
| 176172 | pMC8b-XI5up | yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-5 |
| 176173 | pMC7-XI5dw | yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-5 |
| 176174 | pMC8b-XII2up | yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XII-2 |
| 176175 | pMC7-XII2dw | yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XII-2 |
| 176176 | pMC8b-XII5up | yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XII-5 |
| 176177 | pMC7-XII5dw | yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XII-5 |
| 176178 | pMC6-spacer | yeast MoClo level-0 part plasmid type 6 containing non-coding DNA spacer for construction of MoClo plasmids without yeast marker |
| 176179 | pMC234r-ccdB/TPK2 | yeast MoClo level-0 part plasmid type 234r containing toxic gene dropout cassette (ccdB and TPK2) |
| 176180 | pMC3-TPK2 | yeast MoClo level-0 part plasmid type 3 containing TPK2 gene |
| 176181 | pMC3-yeGFP | yeast MoClo level-0 part plasmid type 3 containing yeast-enhanced GFP |
| 176182 | pMC-Ura-Cen-lvl1 | preassembled yeast MoClo level-1 backbone with GFP dropout and URA3 marker |
| 176183 | pMG343 | preassembled yeast MoClo level-1 backbone with toxic gene dropout cassette (ccdB and TPK2) and URA3 marker |
| 176184 | pMCL8 | gRNA cloning cassette with RPR1p-tetO promoter and terminator RPT1t, RPR1p-tetO-GFP-scRNA-RPR1t |
| 176185 | pMCL8_28bp | plasmid containing scaffold gRNA for Cas9 followed by a 28bp Csy4 recognition sequence, RPR1p-tetO-GFP-scRNA-28bpCsy4-RPR1t |
| 176186 | pMCL9 | 2 mu cloning vector for single or multiplexed gRNAs, SNR52p-sfGFP-scRNA-SUP4t-CYC1t |