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Cryo-EM structure determination of small proteins by nanobody-binding scaffolds (Legobodies).

Wu X, Rapoport TA
Proc Natl Acad Sci U S A. 2021 Oct 12;118(41). pii: 2115001118. doi: 10.1073/pnas.2115001118. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
176075pCAG-Fab_8D3_2_H-H6Express His6 tagged heavy chain of Fab_8D3_2 in mammalian cells
176076pCAG-Fab_8D3_2_LExpress light chain of Fab_8D3_2 in mammalian cells
176077pET28 MBP_PrA/G-H6Express His6 tagged MBP_PrA/G in Escherichia coli

Antibodies from Article