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A feedback loop between lamellipodial extension and HGF-ERK signaling specifies leader cells during collective cell migration.

Hino N, Matsuda K, Jikko Y, Maryu G, Sakai K, Imamura R, Tsukiji S, Aoki K, Terai K, Hirashima T, Trepat X, Matsuda M
Dev Cell. 2022 Oct 10;57(19):2290-2304.e7. doi: 10.1016/j.devcel.2022.09.003. Epub 2022 Sep 28. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
176029pPBbsr2-Raichu-454HRasCTA FRET biosensor for HRas activity
176030pPBpuro-miRFP670-HRasCTEncoding membrane-targeted miRFP670
176031pX459-dMETA knock-out vector for dog MET
176032pX459-dCTNNA1A knock-out vector for dog CTNNA1 (alpha-1-catenin)
176033pX459-dTLN1 exonA vector for the CRISPR-Cas9 system targeting an exon of dog TLN1 (talin1)
176224pX459-dTLN1 intronA vector for the CRISPR-Cas9 system targeting an intron of dog TLN1 (talin1)
186703pPBbleo-miRFP703-eDHFR(69K6)-Tiam1(DH-PH)Mammalian expression of Tiam1(DH-PH) fused to miRFP703-eDHFR(69K6)
186704pPBpuro-mCherry-HRasCTEncoding membrane-targeted mCherry

Antibodies from Article