Inducible transgene expression in PDX models in vivo identifies KLF4 as a therapeutic target for B-ALL.
Liu WH, Mrozek-Gorska P, Wirth AK, Herold T, Schwarzkopf L, Pich D, Volse K, Melo-Narvaez MC, Carlet M, Hammerschmidt W, Jeremias I
Biomark Res. 2020 Sep 16;8:46. doi: 10.1186/s40364-020-00226-z. eCollection 2020.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 176027 | pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP | construct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometry |
| 176028 | pCDH-EF1a-tetR-T2A-iRFP | construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome iRFP720 |
| 176312 | pCDH-EF1a-tetR-T2A-tSapphire | construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome t-Sapphire |