An Agrobacterium-mediated stable transformation technique for the hornwort model Anthoceros agrestis.
Frangedakis E, Waller M, Nishiyama T, Tsukaya H, Xu X, Yue Y, Tjahjadi M, Gunadi A, Van Eck J, Li FW, Szovenyi P, Sakakibara K
New Phytol. 2021 Jun 2. doi: 10.1111/nph.17524.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 185750 | L2-AA007 | Vector to introduce HygR and eGFP under control of p35S into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag. |
| 185751 | L2-AA012 | Vector to introduce eGFP under control of pAaEF1a into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag. |
| 185752 | L1-AA027 | Level 1 loop assembly vector containing a transcriptional unit with a Hygromycin resistance cassette under the control of the A. agrestis EF1a promoter (pAaEF1a). |
| 185753 | L0-AA018 | Level 0 loop assembly vector containing the Anthoceros agrestis Tip1;1 promoter (pAaTip1;1). |
| 185754 | L0-AA008 | Level 0 loop assembly vector containing the Anthoceros agrestis EF1a promoter (pAaEF1a). |