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Addgene

A time-resolved, multi-symbol molecular recorder via sequential genome editing.

Choi J, Chen W, Minkina A, Chardon FM, Suiter CC, Regalado SG, Domcke S, Hamazaki N, Lee C, Martin B, Daza RM, Shendure J
Nature. 2022 Jul 6. pii: 10.1038/s41586-022-04922-8. doi: 10.1038/s41586-022-04922-8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
175808piggyBAC-5xTAPE-1-BlastREncodes 5xTAPE-1 construct that can be integrated using piggyBAC system
179157PB-5'-ENGRAMpiggyBac plasmid for 5' ENGRAM recorder
183790Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBCA single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.

Antibodies from Article