A time-resolved, multi-symbol molecular recorder via sequential genome editing.
Choi J, Chen W, Minkina A, Chardon FM, Suiter CC, Regalado SG, Domcke S, Hamazaki N, Lee C, Martin B, Daza RM, Shendure J
Nature. 2022 Jul 6. pii: 10.1038/s41586-022-04922-8. doi: 10.1038/s41586-022-04922-8.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
175808 | piggyBAC-5xTAPE-1-BlastR | Encodes 5xTAPE-1 construct that can be integrated using piggyBAC system |
179157 | PB-5'-ENGRAM | piggyBac plasmid for 5' ENGRAM recorder |
183790 | Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC | A single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape. |