Design of functionalised circular tandem repeat proteins with longer repeat topologies and enhanced subunit contact surfaces.
Hallinan JP, Doyle LA, Shen BW, Gewe MM, Takushi B, Kennedy MA, Friend D, Roberts JM, Bradley P, Stoddard BL
Commun Biol. 2021 Oct 29;4(1):1240. doi: 10.1038/s42003-021-02766-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 175793 | tcTRP9 | de novo thick circular tandem repeat proteins with 9 repeats |
| 175794 | tcTRP9sub3 | de novo thick circular tandem repeat protein trimer formed from 3-repeat monomers |
| 175795 | tcTRP24sub6 | de novo thick circular tandem repeat protein tetramer formed from 6-repeat monomers |
| 175796 | tcTRP24sub8 | de novo thick circular tandem repeat protein trimer formed from 8-repeat monomers |
| 175797 | tcTRP24sub8_Cap | de novo thick circular tandem repeat protein capped to prevent multimerization |
| 175798 | tcTRP24sub8_SS | de novo thick circular tandem repeat protein trimer formed from 8-repeat monomers containing disulfide 'staples' |
| 175799 | tcTRP24sub8_SH2sub6 | de novo thick circular tandem repeat protein trimer formed from 8-repeat monomers containing 2 copies of human Nck2 SH2 |
| 175800 | SH2-free | Human Nck2 SH2 domain |