An Inactive Dispersin B Probe for Monitoring PNAG Production in Biofilm Formation.
Eddenden A, Kitova EN, Klassen JS, Nitz M
ACS Chem Biol. 2020 May 15;15(5):1204-1211. doi: 10.1021/acschembio.9b00907. Epub 2020 Jan 21.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 175783 | pET29b-eGFP-dspB(E184Q)-6xHis | Plasmid for overexpression of recombinant His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG. |
| 175784 | pET29b-AviTag-eGFP-dspB(E184Q)-6xHis | Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG. |
| 175788 | pET29b-AviTag(K10A)-eGFP-dspB(E184Q)-6xHis | Plasmid for overexpression of recombinant AviTagged(nonfunctional), His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG. |
| 175801 | pET29b-AviTag-eGFP-dspB(E184Q W330Y)-6xHis | Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG. |
| 175802 | pET29b-dspB(E184Q)-6xHis | Plasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG. |