Quantitative proteomics reveals the selectivity of ubiquitin-binding autophagy receptors in the turnover of damaged lysosomes by lysophagy.
Eapen VV, Swarup S, Hoyer MJ, Paulo JA, Harper JW
eLife. 2021 Sep 29;10. pii: 72328. doi: 10.7554/eLife.72328.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 175751 | pHAGE-FLAG-APEX2-GABARAPL2 | Lentiviral vector expressing N-terminally tagged APEX2-FLAG-GABARAPL2 |
| 175752 | pHAGE-FLAG-APEX2-GABARAPL2Y49A/L50A | Lentiviral vector expressing N-terminally tagged APEX2-FLAG-GABARAPL2Y49A/L50A |
| 175753 | pHAGE-FLAG-APEX2-MAP1LC3B | Lentiviral vector expressing N-terminally tagged APEX2-FLAG-MAP1LC3B |
| 175754 | pHAGE-FLAG-APEX2-MAP1LC3BK51A | Lentiviral vector expressing N-terminally tagged APEX2-FLAG-MAP1LC3B K51A |
| 175755 | pHAGE-FLAG-APEX2-LGALS1 | Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS1 |
| 175756 | pHAGE-FLAG-APEX2-LGALS3 | Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS3 |
| 175757 | pHAGE-FLAG-APEX2-GFP | Lentiviral vector expressing N-terminally tagged APEX2-FLAG-GFP (Control for APEX experiments) |
| 175758 | pHAGE-FLAG-APEX2-LGALS8 | Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS8 |
| 175759 | pHAGE-FLAG-APEX2-CALCOCO2 | Lentiviral vector expressing N-terminally tagged APEX2-FLAG-CALCOCO2/NDP52 |
| 175760 | pHAGE-FLAG-APEX2-OPTN | Lentiviral vector expressing N-terminally tagged APEX2-FLAG-OPTN |
| 175761 | pHAGE-FLAG-APEX2-TAX1BP1 | Lentiviral vector expressing N-terminally tagged APEX2-FLAG-TAX1BP1 |
| 175762 | pHAGE-eGFP-OPTN D474N | Lentiviral vector expressing N-terminally tagged eGFP-OPTN D474N |
| 175763 | pHAGE-eGFP-OPTN S473A 513A | Lentiviral vector expressing N-terminally tagged eGFP-OPTN S473A 513A |
| 175764 | pHAGE-eGFP-OPTN S513A | Lentiviral vector expressing N-terminally tagged eGFP-OPTN S513A |
| 175765 | pHAGE-eGFP-OPTN E50K | Lentiviral vector expressing N-terminally tagged eGFP-OPTN E50K |
| 175766 | pHAGE-eGFP-TAX1BP1 | Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 |
| 175767 | pHAGE-eGFP-TAX1BP1 A114Q | Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 A114Q |
| 175768 | pHAGE-eGFP-TAX1BP1 SKICH (1-140D) | Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 SKICH (1-140D) |
| 175769 | pHAGE-eGFP-TAX1BP1 V192S | Lentiviral vector expressing N-terminally tagged GFP-TAX1BP1 V192S |
| 175770 | pHAGE-eGFP-TAX1BP1 Q770A E774K | Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 Q770A E774K |
| 175771 | pHAGE-eGFP-TAX1BP1 632-639D | Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 632-639D |
| 175772 | pHAGE-eGFP-TAX1BP1 Y635A | Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 Y635A |
| 175773 | pHAGE-eGFP-TAX1BP1 N637A | Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 N637A |
| 175774 | pHAGE-eGFP-TAX1BP1 CC1D | Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 CC1D |
| 175775 | pHAGE-eGFP-TAX1BP1 CC2D | Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 CC2D |
| 175776 | pHAGE-eGFP-TAX1BP1 CC3D | Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 CC3D |
| 175777 | pSMART Tmem192-3X HA (targeting vector for genomic tagging) | pSMART Tmem192-3X HA (targeting vector for genomic tagging) |
| 175778 | pAC150-RFP-EGFP-LGALS3 | PiggyBac Vector expressing GFP-RFP-LGALS3 |
| 175779 | pAC150-RFP-EGFP-LGALS3 R186S | PiggyBac Vector expressing GFP-RFP-LGALS3 R186S |
| 175780 | pHAGE-mKeima-LGALS3 | Lentiviral vector expressing N-terminally tagged mKeima-LGALS3 (Lyso-Keima), for quantitative assessment of lysophagic flux |