Engineered HaloTag variants for fluorescence lifetime multiplexing.
Frei MS, Tarnawski M, Roberti MJ, Koch B, Hiblot J, Johnsson K
Nat Methods. 2021 Dec 16. pii: 10.1038/s41592-021-01341-x. doi: 10.1038/s41592-021-01341-x.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 169324 | pET51b(+)_HaloTag9 | Expression of HaloTag9 in bacterial cells |
| 169325 | pCDNA5/FRT/TO_HaloTag7_T2A_EGFP | Expression of HaloTag7 in mammalian cells |
| 169326 | pCDNA5/FRT/TO_HaloTag9_T2A_EGFP | Expression of HaloTag9 in mammalian cells |
| 169327 | pCDNA5/FRT/TO_CEP41-HaloTag7_T2A_EGFP | Expression of CEP41-HaloTag7 in mammalian cells |
| 169328 | pCDNA5/FRT/TO_CEP41_HaloTag9_T2A_EGFP | Expression of HaloTag9 located at the microtubules of mammalian cells |
| 169329 | pCDNA5/FRT/TO_H2B_HaloTag7 | Expression of H2B-HaloTag7 in mammalian cells |
| 169330 | pCDNA5/FRT/TO_TOMM20_HaloTag7 | Expression of TOMM20-HaloTag7 in mammalian cells |
| 169331 | pCDNA5/FRT/TO_CEP41_HaloTag9 | Expression of HaloTag9 located at the microtubules of mammalian cells |
| 169332 | pCDNA5/FRT/TO_H2B_HaloTag9_T2A_EGFP | Nuclear expression of HaloTag9 in mammalian cells |
| 169333 | pCDNA5/FRT/TO_TOMM20_HaloTag9_T2A_EGFP | Expression of HaloTag9 at the mitochondrial surface of mammalian cells |
| 169334 | pCDNA5/FRT/TO_H2B_HaloTag9 | Nuclear expression of HaloTag9 in mammalian cells |
| 169335 | pCDNA5/FRT/TO_TOMM20_HaloTag9 | Expression of HaloTag9 at the mitochondrial surface of mammalian cells |
| 169336 | pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(30-120) | Expression of the LT-Fucci(SA) in mammalian cells |
| 169337 | pCDNA5/FRT/Halo-Tag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-100)Cy+ | Expression of the LT-Fucci(SCA) in mammalian cells |
| 169338 | pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-110)Cy- | Expression of the LT-Fucci(CA) in mammalian cells |
| 175519 | pET-51b-HaloTag11 | Expression of HaloTag11 in bacterial cells |
| 175520 | pET-51b-HaloTag10 | Expression of HaloTag10 in bacterial cells |
| 175521 | pcDNA5/FRT/TO_H2B-HaloTag10-T2A-EGFP | Nuclear expression of HaloTag10 in mammalian cells |
| 175522 | pcDNA5/FRT/TO_H2B-HaloTag11-T2A-EGFP | Nuclear expression of HaloTag11 in mammalian cells |
| 175523 | pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9 | Co-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) in mammalian cells |
| 175524 | pcDNA5/FRT/TO_Beta4Gal-HaloTag7-T2A-Lyn11-SNAPf | Co-expression of HaloTag7 (gogli) and SNAPf-tag (membrane inner leaflet) in mammalian cells |
| 175525 | pcDNA5/FRT/TO_H2B-HaloTag10-T2A-Tomm20-HaloTag9 | Co-expression of HaloTag10 (nuclear) and 9 (mitochondrial surface) in mammalian cells |
| 175526 | pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7 | Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells |
| 175527 | pcDNA5/FRT/TO_NES_HaloTag7 | Strict cytosolic expression of HaloTag7 in mammalian cells |
| 175528 | pcDNA5/FRT/TO_HaloTag7_LamB1 | Expression of HaloTag7 at the nuclear lamina of the mammalian cells |
| 175529 | pcDNA5/FRT/TO_[Cox8a]x2_HaloTag7 | Expression of HaloTag7 at the inner membrane of mitochondria in mammalian cells |
| 175530 | pcDNA5/FRT/TO_CalR_HaloTag7_KDEL | Expression of HaloTag7 in the endoplasmid reticulum of mammalian cells |
| 175531 | pcDNA5/FRT/TO_LAMP1_HaloTag7 | Lyzosomal expression of HaloTag7 in mammalian cells |
| 175532 | pcDNA5/FRT/TO_HaloTag7_SKL | Peroxysomal expression of HaloTag7 in mammalian cells |
| 175533 | pcDNA5/FRT/TO_Lyn11-HaloTag7 | Expression of HaloTag7 at the inner leaflet of the plasma membrane of mammalian cells |
| 175534 | pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb | Expression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cells |
| 175535 | pcDNA5/FRT/TO_Lifeact_HaloTag7 | Expression of HaloTag7 at the actin filaments of mammalian cells |
| 175536 | pcDNA5/FRT/TO_Beta4Gal-HaloTag7 | Expression of HaloTag7 at the at the Gogli apparatus of the mammalian cells |
| 175537 | pcDNA5/FRT/TO_NES_HaloTag9 | Strict cytosolic expression of HaloTag9 in mammalian cells |
| 175538 | pcDNA5/FRT/TO_HaloTag9_LamB1 | Expression of HaloTag9 at the nuclear lamina of the mammalian cells |
| 175539 | pcDNA5/FRT/TO_[Cox8a]x2_HaloTag9 | Expression of HaloTag9 at the inner membrane of mitochondria in mammalian cells |
| 175540 | pcDNA5/FRT/TO_CalR_HaloTag9_KDEL | Expression of HaloTag9 in the endoplasmid reticulum of mammalian cells |
| 175541 | pcDNA5/FRT/TO_LAMP1_HaloTag9 | Lyzosomal expression of HaloTag9 in mammalian cells |
| 175542 | pcDNA5/FRT/TO_HaloTag9_SKL | Peroxysomal expression of HaloTag9 in mammalian cells |
| 175543 | pcDNA5/FRT/TO_Lyn11-HaloTag9 | Expression of HaloTag9 at the inner leaflet of the plasma membrane of mammalian cells |
| 175544 | pcDNA5/FRT/TO_IgKchL_HA_HaloTag9_myc_PDGFRtmb | Expression of HaloTag9 at the outer leaflet of the plasma membrane of mammalian cells |
| 175545 | pcDNA5/FRT/TO_Lifeact_HaloTag9 | Expression of HaloTag9 at the actin filaments of mammalian cells |
| 175546 | pcDNA5/FRT/TO_b4g-HaloTag9 | Expression of HaloTag9 at the at the Gogli apparatus of the mammalian cells |
| 175547 | pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9 | Co-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cells |