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Opher Gileadi Lab: TREAT-AD plasmids 2021

Gileadi, et al
unpublished

Plasmids from Article

ID Plasmid Purpose
175448CD44I-c001Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N172.
175449CD44I-c011Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N178. Used for crystallography.
175450CD44I-c013Protein expression/secretion in mammalian cells. CD44 Link module (exons 2-5), A20-P220.
175451CD44I-c014Protein expression/secretion in mammalian cells. CD44 Link module (exons 2-5), A20-P220. Can be biotinylated.
175452CD44MMA-c002Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, Q23-D172. Used for crystallography.
175453CD44U-c010Protein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649.
175454CD44U-c011Protein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649. Can be biotinylated.
175455CD44Z-c011Protein expression/secretion in mammalian cells. CD44s (standard form; exons 2-5, 15-16), A20-E649.
175456CNN3A-c002Protein expression in bacterial cells. Entire CDS, M1-Y329.
175457CNN3A-c007Protein expression in bacterial cells. Lacking N-terminal 10 residues, G11-Y329.
175458EPB41L3A-c000Protein expression in bacterial cells. Entire CDS, M1-D1087.
175459EPB41L3A-c001Protein expression in bacterial cells. FERM domain, M1-L390.
175460EPB41L3A-c021Protein expression in bacterial cells. FERM domain, P107-L390. Used for crystallography (PDB:6IBE).
175461EZRA-c004Protein expression in bacterial cells. FERM domain, M1-Q322.
175463MDKA-c009Protein expression/secretion in mammalian cells. Entire CDS with ectopic signal peptide, V21-D143
175464MDKA-c014Protein expression/secretion in mammalian cells. Entire CDS with ectopic signal peptide, V21-D143. Fused to IgG Fc.
175465MSNA-c000Protein expression in bacterial cells. Entire CDS, M1-M577
175467MSNA-c007Protein expression in bacterial cells. FERM domain, M1-D298. Used for NMR studies.
175469MSNA-c023Protein expression in bacterial cells. FERM domain, M1-E346. Can be biotinylated.
175472NF2A-c001Protein expression in bacterial cells. FERM domain, M1-R338.
175473PRDX1A-c001Protein expression in bacterial cells. Entire CDS, M1-K199. Can be biotinylated.
175474PRDX6A-c001Protein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.
175475RAB5AA-c003Protein expression in bacterial cells. Entire CDS, M1-N215.
175476RAB5AA-c006Protein expression in bacterial cells. GTPase domain, G15-N184. Can be used for crystallography.
175477RABEP1A-c019Protein expression in insect cells. Entire CDS, M1-T862. Codon optimised.
175478RABGEF1A-c014Protein expression in insect cells. Entire CDS, M1-G491. Codon optimised.
175479RDXB-c004Protein expression in bacterial cells. FERM domain, M1-A321.
175480SDCBPA-c000Protein expression in bacterial cells. Entire CDS, M1-V288.
175482SDCBPA-c010Protein expression in bacterial cells. PDZ1 and PDZ2 domains, A106-V288. Can be used for crystallography.
175483SDCBPA-c011Protein expression in bacterial cells. PDZ1 and PDZ2 domains (truncated at C-terminus), A106-F275.
175484SDCBPA-c020Protein expression in bacterial cells. PDZ1 and PDZ2 domains, G111-V288.
175485SDCBPA-c021Protein expression in bacterial cells. PDZ1 and PDZ2 domains (truncated at C-terminus), G111-F275.
175486SNX32A-c014Protein expression in bacterial cells. PX domain, S17-M197
175487STX4AA-c005Protein expression in bacterial cells. Coiled-coil domains, M1-L275.
175488SYKA-c014Protein expression in insect cells. Entire CDS, M1-N635. Codon optimised.
175491XX01CAPN1A-c001 Protein expression in bacterial cells. Entire CDS, M1-A714 (CAPN1); EF hand 1-5, H86-S268 (CAPNS1). CAPN1 can be biotinylated. Codon optimised.
175492XX01CAPN2A-c001 Protein expression in bacterial cells. Entire CDS, M1-L700 (CAPN2); EF hand 1-5, H86-S268 (CAPNS1). CAPN2 can be biotinylated. Codon optimised.
175493YSCD44A-c002Protein expression in bacterial cells. Single chain Fv antibody to human CD44 Link domain. Can be used for crystallography.

Antibodies from Article