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Mechanisms that ensure speed and fidelity in eukaryotic translation termination.

Lawson MR, Lessen LN, Wang J, Prabhakar A, Corsepius NC, Green R, Puglisi JD
Science. 2021 Aug 20;373(6557):876-882. doi: 10.1126/science.abi7801. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
175413Sc eRF1 (WT)Encodes His-MBP-TEV-WT eRF1
175414Sc NybbR eRF1 (WT)Encodes His-MBP-TEV-ybbR-WT eRF1
175415Sc NybbR eRF1 (G180A)Encodes His-MBP-TEV-ybbR-G180A eRF1
175418Sc eRF3∆165N (WT)Encodes His-MBP-TEV-WT eRF3∆165N
175419Sc NybbR eRF3∆165N (WT)Encodes His-MBP-TEV-ybbR-WT eRF3∆165N
175420Sc NybbR eRF3∆165N (H348E)Encodes His-MBP-TEV-ybbR-H348E eRF3∆165N

Antibodies from Article