Design and Properties of Genetically Encoded Probes for Sensing Macromolecular Crowding.
Liu B, Aberg C, van Eerden FJ, Marrink SJ, Poolman B, Boersma AJ
Biophys J. 2017 May 9;112(9):1929-1939. doi: 10.1016/j.bpj.2017.04.004.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172928 | pRSETA_crE6 | Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 1 helix and 2 GSG linkers |
| 172929 | pRSETA_crE6G2 | Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices, and a 6AA GSG domain |
| 172930 | pRSETA_crG18 | Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 18x GSG |