A sensor for quantification of macromolecular crowding in living cells.
Boersma AJ, Zuhorn IS, Poolman B
Nat Methods. 2015 Mar;12(3):227-9, 1 p following 229. doi: 10.1038/nmeth.3257. Epub 2015 Feb 2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172927 | pRSETA_crGE | Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains. |
| 172933 | pcDNA3.1_crGE | Crowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains. |