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Plasmid replication based on the T7 origin of replication requires a T7 RNAP variant and inactivation of ribonuclease H.

Becker K, Meyer A, Roberts TM, Panke S
Nucleic Acids Res. 2021 Jul 13. pii: 6320377. doi: 10.1093/nar/gkab596. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
175356pT7RNAP-Q737PSupport plasmid for T7_ori plasmids

Antibodies from Article