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Visualizing looping of two endogenous genomic loci using synthetic zinc-finger proteins with anti-FLAG and anti-HA frankenbodies in living cells.

Liu Y, Zhao N, Kanemaki MT, Yamamoto Y, Sadamura Y, Ito Y, Tokunaga M, Stasevich TJ, Kimura H
Genes Cells. 2021 Nov;26(11):905-926. doi: 10.1111/gtc.12893. Epub 2021 Sep 20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
175291iRFP713-N2-Frankenbody-FlagVisualize endogeneous loci coupled with ZF probes harboring 10xFLAG tag
175292mCherry-N2-Frankenbody-HAVisualize endogeneous loci coupled with ZF probes harboring 10xHA tag
175293pCMV-Frankenbody-FLAG-mEGFPTrack mature and nascent FLAG tagged proteins in live cells
175294pCMV-Frankenbody-FLAG-mRuby2Track mature and nascent FLAG tagged proteins in live cells
175295pCMV-Frankenbody-FLAG-SNAPTrack mature FLAG tagged proteins in live cells
175296pCMV-Frankenbody-FLAG-HaloTrack mature and nascent FLAG tagged proteins in live cells

Antibodies from Article