Anterograde transneuronal tracing and genetic control with engineered yellow fever vaccine YFV-17D.
Li E, Guo J, Oh SJ, Luo Y, Oliveros HC, Du W, Arano R, Kim Y, Chen YT, Eitson J, Lin DT, Li Y, Roberts T, Schoggins JW, Xu W
Nat Methods. 2021 Nov 25. pii: 10.1038/s41592-021-01319-9. doi: 10.1038/s41592-021-01319-9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 175273 | pAAV-DIO-NS1 | AAV vector mediating Cre-depenent expression of NS1 gene of YFV-17D |
| 175274 | pAAV-rtTA | AAV vector mediating neuron-specific expression of rtTA |
| 175275 | pAAV-SynaptoTAG2 | AAV vector for mapping synaptic projections of infected neurons; expresses tdTomato to reveal neuronal soma and axons, and expresses EGFP fused to Synaptobrevin-2 to track synaptic terminals. |
| 175276 | AAV-Syn-NS1 | AAV vector mediating bicistronic expression of NS1 gene of YFV-17D and dTomato with NLS |
| 175277 | AAV-TRE-C-prM-E-NS1 | AAV vector mediating inducible expression of 4 genes (the C-prM-E-NS1) of YFV-17D |
| 175278 | AAV-TRE-NS1-dTomato | AAV vector mediating inducible bicistronic expression of NS1 gene of YFV-17D and dTomato |
| 175279 | AAV-TRE-NS1NF | AAV vector mediating inducible expression of the NS1 gene of YFV-17D |
| 175280 | AAV-tTA | AAV vector mediating neuron-specific expression of tTA |
| 175281 | FUW-C-prM-E-NS1 | lentiviral vector mediating bicistronic expression of the 4 genes of YFV-17D (C-prM-E-NS1) and EGFP. |
| 179952 | YFVdelNS1-Cre | pCCI vector encoding YFV-17D lacking nonstructural protein 1 (NS1) but with Cre |
| 179953 | YFVdelCME-mVenus | pCCI vector encoding YFV-17D lacking C, prM, E proteins but with mVenus |