In Situ Visualization of Axon Growth and Growth Cone Dynamics in Acute Ex Vivo Embryonic Brain Slice Cultures.
Alfadil E, Bradke F, Dupraz S
J Vis Exp. 2021 Oct 14;(176). doi: 10.3791/63068.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 175257 | pCAG-loxpSTOPloxp-lyn-NeonGreen | membrane target protein labelling |
| 175258 | pTub-alpha1-iLifeact-NeonGreen | F-actin structures labelling |
| 175259 | pCAG-lox-rox-STOP-rox-lynScarlet-lox-Lyn-NeonGreen | dual plasmid labelling membrane target protein of different neuronal population with different flurophore |
| 175261 | pTub-alpha1-EB3-NeonGreen-T2A-iLifeact-mCherry | labelling of microtubule plus end and F-actin structures |
| 175263 | pTub-alpha-EB3-NeonGreen | labelling of microtubule plus end and F-actin structures |
| 175437 | pTub-alpha1-Lifeact-GFP | F-actin structures labelling |
| 175438 | pCAG-lox-rox-STOP-rox-tRFP-pA-lox-ZsGreen-pA | dual plasmid labelling cells of different neuronal population with different fluorophores |