Mechanistic dissection of increased enzymatic rate in a phase-separated compartment.
Peeples W, Rosen MK
Nat Chem Biol. 2021 Jun;17(6):693-702. doi: 10.1038/s41589-021-00801-x. Epub 2021 May 25.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 175232 | polyPRM5 | Bacterial expression and purification, mixed with polySH3 to form condensates |
| 175233 | FRB-polySH33 | Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin |
| 175234 | FRB-polySH35 | Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin |
| 175235 | EGFP-RanGAP | Bacterial expression and purification, high affinity SUMOylation substrate |
| 175236 | FKBP-EGFP-RanGAP | Bacterial expression and purification, high affinity SUMOylation substrate that can recruited to FRB with rapamycin |
| 175237 | EGFP-RanGAP* | Bacterial expression and purification, low affinity SUMOylation substrate, point mutation F562A reduces affinity for E2, increasing the Km |
| 175238 | FKBP-EGFP-RanGAP* | Bacterial expression and purification, low affinity SUMOylation substrate that can recruited to FRB with rapamycin, point mutation F562A reduces affinity for E2, increasing the Km |
| 175239 | ShadowG-SUMO1 | Bacterial expression and purification, conjugatable SUMO for SUMOylation |
| 175240 | SAE2-mGarnet | Bacterial expression and purification, combined with SAE1 to form the E1 enzyme heterodimer |
| 175241 | SAE1 | Bacterial expression and purification, combined with SAE2 to form the E1 enzyme heterodimer |
| 175242 | mCherry-E2 | Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation |
| 175243 | mCherry-FKBP-E2 | Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin, CyPet acts as a FRET donor to Ypet |
| 175244 | CyPet-FKBP-E2 | Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin |
| 175245 | FKBP-YPet-RanGAP* | Bacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPet |
| 175246 | EGFP-PML peptide | Bacterial expression and purification, low affinity SUMOylation substrate |
| 175247 | FKBP-EGFP-PML peptide | Bacterial expression and purification, low affinity SUMOylation substrate with a relatively high Km, can be recruited to FRB with rapamycin |
| 175792 | FRB-polySH33-EGFP | Bacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantification |