Exploiting Polyploidy for Markerless and Plasmid-Free Genome Engineering in Cyanobacteria.
Jones CM, Parrish S, Nielsen DR
ACS Synth Biol. 2021 Sep 17;10(9):2371-2382. doi: 10.1021/acssynbio.1c00269. Epub 2021 Aug 17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 175052 | pCJ111 | rbcLXS7002 integrative plasmid expresses CRE recombinase as a translational fusion to rbcL |
| 175054 | pCJ155 | psbEFLJ integrative plasmid expresses CRE recombinase from the rbcL promoter uses zeocin to integrate |
| 175055 | pCJ162 | lox66-72 flanked gentamycin resistance cassette integrates into aquI integration site |
| 175056 | pCJ163 | rox flanked gentamycin resistance cassette integrates into A2542 integration site |
| 175057 | pCJ164 | rbcLXS7002 integrative plasmid expresses DRE recombinase as a translational fusion to rbcL |
| 175058 | pZD-CJ1111 | rbcLXS6803 integrative plasmid expresses CRE recombinase as a translational fusion to rbcL6803 |