Structure of the human clamp loader reveals an autoinhibited conformation of a substrate-bound AAA+ switch.
Gaubitz C, Liu X, Magrino J, Stone NP, Landeck J, Hedglin M, Kelch BA
Proc Natl Acad Sci U S A. 2020 Sep 22;117(38):23571-23580. doi: 10.1073/pnas.2007437117. Epub 2020 Sep 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 175043 | pCDF-1b-RFC140ΔN555 | Overexpression of human (Rfc1) p140ΔN555 in E.coli |
| 175049 | p36-p37-p38-p40-pET-Duet-1 | Overexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coli |
| 175050 | p36-p37-p38-p40-pET-Duet-1-Rfc3-3KtoA | Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli |
| 175051 | p36-p37-p38-p40-pET-Duet-1-Rfc3-T76D | Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli |