Deletion and replacement of long genomic sequences using prime editing.
Jiang T, Zhang XO, Weng Z, Xue W
Nat Biotechnol. 2021 Oct 14. pii: 10.1038/s41587-021-01026-y. doi: 10.1038/s41587-021-01026-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174851 | PE-Cas9 | Express PE-Cas9 in mammalian cells |
| 174852 | HEK3_pegF_18bp | Express forward pegRNA mediating 991-bp deletion and 18-bp insertion |
| 174853 | HEK3_pegR_18bp | Express reverse pegRNA mediating 991-bp deletion and 18-bp insertion |
| 174854 | HEK3_pegF_44bp | Express forward pegRNA mediating 991-bp deletion and 44-bp insertion |
| 174855 | HEK3_pegR_44bp | Express forward pegRNA mediating 991-bp deletion and 44-bp insertion |
| 174856 | HEK3_pegF_60bp | Express forward pegRNA mediating 991-bp deletion and 60-bp insertion |
| 174857 | HEK3_pegR_60bp | Express reverse pegRNA mediating 991-bp deletion and 60-bp insertion |