A repackaged CRISPR platform increases homology-directed repair for yeast engineering.
Ploessl D, Zhao Y, Cao M, Ghosh S, Lopez C, Sayadi M, Chudalayandi S, Severin A, Huang L, Gustafson M, Shao Z
Nat Chem Biol. 2021 Oct 28. pii: 10.1038/s41589-021-00893-5. doi: 10.1038/s41589-021-00893-5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174837 | YL-CashGem-BAT1-URA3-LINEAR | pRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolytica |
| 174838 | KM-CashGem-Xyl2-URA3-LINEAR | pRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianus |
| 174839 | HP-Cas9hGem-Ade2-LEU2-LINEAR | pRS414 backbone containing LINEAR fragment targeting ADE2 in H. polymorpha |
| 174840 | pCashGem-Xyl2-URA3-LINEAR | pRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitis |