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Optimization of a bacterial three-hybrid assay through in vivo titration of an RNA-DNA adapter protein.

Wang CD, Mansky R, LeBlanc H, Gravel CM, Berry KE
RNA. 2021 Apr;27(4):513-526. doi: 10.1261/rna.077404.120. Epub 2021 Jan 26. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
174786p35u4Constitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system

Antibodies from Article