MPP8 is essential for sustaining self-renewal of ground-state pluripotent stem cells.
Muller I, Moroni AS, Shlyueva D, Sahadevan S, Schoof EM, Radzisheuskaya A, Hojfeldt JW, Tatar T, Koche RP, Huang C, Helin K
Nat Commun. 2021 May 24;12(1):3034. doi: 10.1038/s41467-021-23308-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 175036 | pRepair-MPP8_mAID-P2A-BFP | Homology Directed Repair construct for C-terminal tagging of mMPP8 with mAID using CRISPR/Cas9 |
| 175037 | pL-U6-sgRNA-SFFV-Puro-P2A-EGFP | Lentiviral delivery of sgRNA. Expresses PuroR and eGFP from SFFV promoter. |
| 175038 | pPB-MPP8_Flag2-Blast | Mammalian expression of full length mMPP8 with C-terminal Flag2 tag |
| 175039 | pPB-MPP8-112–858_Flag2-Blast | Mammalian expression of mMPP8 (E112 to Q858) with C-terminal Flag2 tag |
| 175040 | pPB-MPP8-1–729_Flag2-Blast | Mammalian expression of mMPP8 (M1 to E729) with C-terminal Flag2 tag |
| 175041 | pPB-MPP8-∆ARD_Flag2-Blast | Mammalian expression of mMPP8 (deletion from D570 to K718) with C-terminal Flag2 tag |