Genetic identification of the functional surface for RNA binding by Escherichia coli ProQ.
Pandey S, Gravel CM, Stockert OM, Wang CD, Hegner CL, LeBlanc H, Berry KE
Nucleic Acids Res. 2020 May 7;48(8):4507-4520. doi: 10.1093/nar/gkaa144.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174662 | pCH6 - pBait-ChiX | arabinose-induced synthesis of a hybrid RNA containing a single MS2 hairpin followed by E coli ChiX inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest. |
| 174663 | pCH1 - pBait-empty | arabinose-induced synthesis of an RNA containing a single MS2 hairpin. Used as a three-hybrid control. For cloning purposes, see plasmid #174662. |
| 174664 | pCW17 - pAdapter | Constitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system. |