Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination.

Enghiad B, Huang C, Guo F, Jiang G, Wang B, Tabatabaei SK, Martin TA, Zhao H
Nat Commun. 2021 Feb 19;12(1):1171. doi: 10.1038/s41467-021-21275-4. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
174653	pBE14	In vivo Cre-lox circularization in E. coli
174654	pBE44	CAPTURE method 15A origin of replication receiver plasmid
174655	pBE45	CAPTURE method receiver plasmid for Streptomyces heterologous expression
174656	pBE48	CAPTURE method BAC origin of replication receiver plasmid
174657	pBE50	CAPTURE method receiver plasmid for Bacillus cloning and heterologous expression
174658	pET28-FnCas12a-TEV	FnCas12a expression plasmid