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Development of mammalian cell logic gates controlled by unnatural amino acids.

Mills EM, Barlow VL, Jones AT, Tsai YH
Cell Rep Methods. 2021 Sep 16;1(6):100073. doi: 10.1016/j.crmeth.2021.100073. eCollection 2021 Oct 25. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
174526PylT(UAG) PylS mCherry-P2A-eGFP(150TAG)Dual-fluorescence reporter for quantifying PylT(UAG) decoding efficiency
174527PylT(CTAG)WT PylS mCherry-P2A-eGFP(CTAG)Dual-fluorescence reporter for quantifying PylT(CTAG)WT decoding efficiency
174528PylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency
1745294xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

Antibodies from Article