A FRET-based method for monitoring structural transitions in protein self-organization.
Wan Q, Mouton SN, Veenhoff LM, Boersma AJ
Cell Rep Methods. 2022 Mar 28;2(3):100184. doi: 10.1016/j.crmeth.2022.100184. eCollection 2022 Mar 28.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 175060 | pQ54-VC | expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ54) in mammalian cells |
| 175061 | pQ39-VC | expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ39) in mammalian cells |
| 175062 | pQ25-VC | expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ25) in mammalian cells |
| 175063 | pVC | expresses mVenus and mCherry pair in mammalian cells |
| 175064 | pQ79 | expresses mutant of the huntingtin exon1 in mammalian cells |
| 175065 | pVC-DNAJB6b | expresses mVenus and mCherry tagged DNAJB6b in mammalian cells |
| 175066 | pVC-FUS-WT | expresses mVenus and mCherry tagged to FUS wildtype in mammalian cells |
| 175067 | pVC-FUS-R521C | expresses mVenus and mCherry tagged to mutant of the FUS (R521C) protein in mammalian cells |
| 175068 | pFUS-WT-VC | expresses FUS wildtype tagged to mVenus and mCherry in mammalian cells |